Detection and characterization of Extended-Spectrum beta-lactamases (ESBLs) in Salmonella enterica strains of healthy food animals in Spain

  1. Riaño, I. 1
  2. Moreno, M.A. 2
  3. Teshager, T. 2
  4. Sáenz, Y. 1
  5. Domínguez, L. 2
  6. Torres, C. 1
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Universidad Complutense de Madrid
    info

    Universidad Complutense de Madrid

    Madrid, España

    ROR 02p0gd045

Revista:
Journal of Antimicrobial Chemotherapy

ISSN: 0305-7453

Año de publicación: 2006

Volumen: 58

Número: 4

Páginas: 844-847

Tipo: Artículo

DOI: 10.1093/JAC/DKL337 PMID: 16935865 SCOPUS: 2-s2.0-33748697409 WoS: WOS:000240587400022 GOOGLE SCHOLAR

Otras publicaciones en: Journal of Antimicrobial Chemotherapy

Objetivos de desarrollo sostenible

Resumen

Objectives: To carry out the characterization of the genes encoding extended-spectrum β-lactamases (ESBLs) and their genetic environments in four expanded-spectrum cephalosporin-resistant Salmonella enterica isolates (serovars: two Virchow, one Enteritidis, one Rissen) recovered during the monitoring programmes performed in Spain by the VAV Network from faecal samples of pigs, poultry and laying hens at the slaughterhouse level. Methods: The presence and characterization of ESBL genes as well as their genetic environments in the four S. enterica isolates were investigated by PCR and sequencing. The presence of other resistance genes was also analysed by PCR and sequencing. Results: Three avian S. enterica isolates (two Virchow and one Enteritidis) harboured the blaCTX-M-9 gene combined with blaTEM-1b. The blaCTX-M-9 gene was included in these three isolates in a class 1 integron with the following 5′→3′ structure: integron 1 variable region (dfrA16-aadA2 gene cassettes)-qacEΔ1-sul1-orf 513-blaCTX-M-9-orf3-like- orf1005. The sul2 gene was also detected in these three blaCTX-M-9-containing isolates and tet (A) in one of them. The two serovar Virchow isolates showed an indistinguishable PFGE pattern, although they were recovered from different animal species (broiler and laying hen). A porcine ESBL-positive isolate (serovar Rissen) harboured the blaSHV-12 gene combined with blaTEM-1b. This blaSHV-12 -containing isolate also harboured the tet (A), aadA and sul1 genes. Conclusions: The emergence of ESBL-producing S. enterica isolates among food animals is described for the first time in Spain, with those of the CTX-M group being the predominant ESBLs detected. © 2006 Oxford University Press.