Evaluation of the osteoclastogenic process associated with RANK / RANK-L / OPG in odontogenic myxomas

  1. Maria del Carmen Gonzalez Galvan 1
  2. Adalberto Mosqueda Taylor 2
  3. Ronell Bologna Molina 3
  4. Amaia Setién Olarra 4
  5. Xabier Marichalar Mendia 4
  6. José Manuel Aguirre Urizar 4
  1. 1 Faculty of Dentistry, National University of Asunción, Paraguay. Scholarship by Itaipú Binacional- Paraguay
  2. 2 Health Care Department, Universidad Autónoma Metropolitana Xochimilco, Ciudad de México, México
  3. 3 Faculty of Dentistry, University of the Republic (UDELAR), Uruguay
  4. 4 UFI 11/25. Department of Stomatology II. Faculty of Medicine and Nursing. University of the Basque Country (UPV/EHU), Leioa, Spain
Show affiliations +
Journal:
Medicina oral, patología oral y cirugía bucal. Ed. inglesa

ISSN: 1698-6946

Year of publication: 2018

Volume: 23

Issue: 3

Pages: 9

Type: Article

DOI: 10.4317/MEDORAL.22372 DIALNET GOOGLE SCHOLAR lock_openOpen access editor

More publications in: Medicina oral, patología oral y cirugía bucal. Ed. inglesa

Abstract

Odontogenic myxoma (OM) is a benign intraosseous neoplasm that exhibits local aggressiveness and high recurrence rates. Osteoclastogenesis is an important phenomenon in the tumor growth of maxillary neoplasms. RANK (Receptor Activator of Nuclear Factor κappa B) is the signaling receptor of RANK-L (Receptor activator of nuclear factor kappa-Β ligand) that activates the osteoclasts. OPG (osteoprotegerin) is a decoy receptor for RANK-L that inhibits pro-osteoclastogenesis. The RANK / RANKL / OPG system participates in the regulation of osteolytic activity under normal conditions, and its alteration has been associated with greater bone destruction, and also with tumor growth. To analyze the immunohistochemical expression of OPG, RANK and RANK-L proteins in odontogenic myxomas (OMs) and their relationship with the tumor size. Eighteen OMs, 4 small (<3 cm) and 14 large (> 3cm) and 18 dental follicles (DF) that were included as control were studied by means of standard immunohistochemical procedure with RANK, RANKL and OPG antibodies. For the evaluation, 5 fields (40x) of representative areas of OM and DF were selected where the expression of each antibody was determined. Descriptive and comparative statistical analyses were performed with the obtained data. There are significant differences in the expression of RANK in OM samples as compared to DF (p = 0.022) and among the OMSs and OMLs (p = 0.032). Also a strong association is recognized in the expression of RANK-L and OPG in OM samples. Activation of the RANK / RANK-L / OPG triad seems to be involved in the mechanisms of bone balance and destruction, as well as associated with tumor growth in odontogenic myxomas.

Bibliographic References

  • Schmidt-Westhausen, A, Becker, J, Schuppan, D, Burkhardt, A, Reichart, PA. (1994). Odontogenic myxoma--characterisation of the extracellular matrix (ECM) of the tumour stroma. Eur J Cancer B Oral Oncol. 30. 377
  • Mosqueda Taylor, A, Ledesma Montes, C, Caballero Sandoval, S, Portilla Robertson, J, Ruíz Godoy Rivera, LM, Meneses García, A. (1997). Odontogenic tumors in Mexico: a collaborative retrospective study of 349 cases. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 84. 672
  • Ochsenius, G, Ortega, A, Godoy, L, Peñafiel, C, Escobar, E. (2002). Odontogenic tumors in Chile: a study of 362 cases. J Oral Pathol Med. 31. 415
  • Ladeinde, AL, Ajayi, OF, Ogunlewe, MO, Adeyemo, WL, Arotiba, GT, Bamgbose, BO. (2005). Odontogenic tumors: a review of 319 cases in a Nigerian teaching hospital. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 99. 191
  • Buchner, A, Merrell, PW, Carpenter, WM. (2006). Relative frequency of central odontogenic tumors: a study of 1,088 cases from Northern California and comparison to studies from other parts of the world. J Oral Maxillofac Surg. 64. 1343
  • Jing, W, Xuan, M, Lin, Y, Wu, L, Liu, L, Zheng, X. (2007). Odontogenic tumours: a retrospective study of 1642 cases in a Chinese population. Int J Oral Maxillofac Surg. 36. 20
  • Sriram, G, Shetty, RP. (2008). Odontogenic tumors: a study of 250 cases in an Indian teaching hospital. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 105. 14-21
  • Luo, HY, Li, TJ. (2009). Odontogenic tumors: a study of 1309 cases in a Chinese population. Oral Oncol. 45. 706
  • Gupta, B, Ponniah, I. (2010). The pattern of odontogenic tumors in a government teaching hospital in the southern Indian state of Tamil Nadu. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 110. 32
  • Osterne, RL, Brito, RG, Alves, AP, Cavalcante, RB, Sousa, FB. (2011). Odontogenic tumors: a 5-year retrospective study in a Brazilian population and analysis of 3406 cases reported in the literature. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 111. 474
  • Siriwardena, BS, Tennakoon, TM, Tilakaratne, WM. (2012). Relative frequency of odontogenic tumors in Sri Lanka: Analysis of 1677 cases. Pathol Res Pract. 208. 225
  • Da-Costa, DO, Maurício, AS, De Faria, PA, Da Silva, LE, Mosqueda Taylor, A, Lourenço, SD. (2012). Odontogenic tumors: a retrospective study of four Brazilian diagnostic pathology centers. Med Oral Patol Oral Cir Bucal. 17. 389
  • Barker, BF. (1999). Odontogenic myxoma. Semin Diagn Pathol. 16. 297-301
  • Martínez Mata, G, Mosqueda Taylor, A, Carlos Bregni, R, De Almeida, OP, Contreras Vidaurre, E, Vargas, PA. (2008). Odontogenic myxoma: clinico-pathological, immunohistochemical and ultrastructural findings of a multicentric series. Oral Oncol. 44. 601
  • Gomes, CC, Diniz, MG, Duarte, AP, Bernardes, VF, Gomez, RS. (2011). Molecular review of odontogenic myxoma. Oral Oncol. 47. 325
  • Simon, EN, Merkx, MA, Vuhahula, E, Ngassapa, D, Stoelinga, PJ. (2004). Odontogenic myxoma: a clinicopathological study of 33 cases. Int J Oral Maxillofac Surg. 33. 333
  • Tekkesin, MS, Mutlu, S, Olgac, V. (2011). The role of RANK/RANKL/OPG signalling pathways in osteoclastogenesis in odontogenic keratocysts, radicular cysts, and ameloblastomas. Head Neck Pathol. 5. 248
  • Wittrant, Y, Théoleyre, S, Chipoy, C, Padrines, M, Blanchard, F, Heymann, D. (2004). RANKL/RANK/OPG: new therapeutic targets in bone tumours and associated osteolysis. Biochim Biophys Acta. 1704. 49-57
  • Da Silva, TA, Batista, AC, Mendonça, EF, Leles, CR, Fukada, S, Cunha, FQ. (2008). Comparative expression of RANK, RANKL, and OPG in keratocystic odontogenic tumors, ameloblastomas, and dentigerous cysts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 105. 333
  • Tekkesın, MS, Mutlu, S, Olgaç, V. (2012). Expressions of bax, bcl-2 and Ki-67 in odontogenic keratocysts (Keratocystic Odontogenic Tumor) in comparison with ameloblastomas and radicular cysts. Turk Patoloji Derg. 28. 49-55
  • Seifert, M, Nelke, KH, Noczyńska, A, Łysenko, L, Kubacka, M, Gerber, H. (2015). Bone markers in craniofacial bone deformations and dysplasias. Postepy Hig Med Dosw. 28. 1176
  • Odell, EW, Adebly, K. (2017). Odontogenic myxoma /myxofibroma. In: El-Naggar AK, Chan JKC, Grandis JR, Takata T, Slootweg PJ. IARC Press. Lyon.
  • Andrade, FR, Sousa, DP, Mendonça, EF, Silva, TA, Lara, VS, Batista, AC. (2008). Expression of bone resorption regulators (RANK, RANKL, and OPG) in odontogenic tumors. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 106. 548
  • Bologna Molina, R, Damián Matsumura, P, Molina Frechero, N. (2011). An easy cell counting method for immunohistochemistry that does not use an image analysis program. Histopathology. 59. 801
  • Wu, PF, Tang, JY, Li, KH. (2015). RANK pathway in giant cell tumor of bone: pathogenesis and therapeutic aspects. Tumour Biol. 36. 495-501
  • Qian, Y, Huang, HZ. (2010). The role of RANKL and MMP-9 in the bone resorption caused by ameloblastoma. J Oral Pathol Med. 39. 592
  • De Matos, FR, De Moraes, M, Das Neves Silva, EB, Galvão, HC, De Almeida Freitas, R. (2013). Immunohistochemical detection of receptor activator nuclear κB ligand and osteoprotegerin in odontogenic cysts and tumors.. J Oral Maxillofac Surg. 71. 1886
  • Bologna-Molina, R, Mosqueda-Taylor, A, Domínguez-Malagón, H, Salazar-Rodríguez, S, Tapia, G, Gónzalez-Gónzalez, R. (2015). Immunolocalization of VEGF-A and orosomucoid-1 in odontogenic myxoma. Rom J Morphol Embryol. 56. 465-473
  • González-Galván, MC, Aguirre-Urizar, JM, Bologna-Molina, R, Farfán-Morales, JE, Gainza-Cirauqui, ML, Marichalar-Mendia, X. (2016). Assessment of CD-105 as an Angiogenic Modulator in Odontogenic Myxomas and Dental Follicles. Int J Surg Pathol. 24. 315
Data source: Dialnet