Comparison of size and protein decoration between extracellular vesicles isolated from adipose tissue and hair follicle mesenchymal stem cells

  1. Kevin Las heras Zapata 15
  2. Felix Miguel Royo Lopez 26
  3. Garcia Vallicrosa C 6
  4. Manuela Igartua Olaechea 135
  5. Edorta Santos Vizcaíno 135
  6. Juan Manuel Falcón Pérez 246
  7. Rosa María Hernández Martín 135
  1. 1 Bioaraba, NanoBioCel Research Group, Vitoria-Gasteiz, Spain
  2. 2 Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), 28029 Madrid, Spain
  3. 3 Biomedical Research Networking Centre in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN).
  4. 4 IKERBASQUE, Basque Foundation for Science, 48013 Bilbao, Spain
  5. 5 NanoBioCel Group, Laboratory of Pharmaceutics, School of Pharmacy (UPV/EHU).
  6. 6 Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Exosomes Laboratory, 48160 Derio, Spain
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Revista:
RESCIFAR Revista Española de Ciencias Farmacéuticas

ISSN: 2660-6356

Año de publicación: 2021

Título del ejemplar: XV CONGRESO DE LA SOCIEDAD ESPAÑOLA DE FARMACIA INDUSTRIA Y GALÉNICA

Volumen: 2

Número: 2

Páginas: 189-190

Tipo: Artículo

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Otras publicaciones en: RESCIFAR Revista Española de Ciencias Farmacéuticas

Resumen

In the current years, extracellular vesicles (EVs) have gained the overall attention in modern regenerative medicine therapeutics. Their demonstrated potential for the treatment of different conditions and their principal role in the efficacy of secretome-based therapies has driven their use. Indeed, mesenchymal stem cells (MSCs)-derived EVs (MSC-EVs) are one the most popular source of EVs due to their largely demonstrated regenerative capacity. Some research groups have performed morphological studies of MSC-EVs by electron microscopy (EM). However, none of them have compared EVs from adipose tissue-derived MSCs (AT-MSCs) — the gold standard in MSCEVs research — and hair follicle-derived MSCs (HF-MSCs) by using Cryo-transmission electron microscopy (TEM) imaging on rapidly-frozen samples. With this technique, it could be potentially reduced the sample damaging and artefacts caused by the addition of heavy metals, dehydration, or fixation steps. We therefore used cryo-TEM to compare, in their near-native state, the morphology and membrane protein decoration of EVs isolated from AT-MSCs and HF-MSCs.

Fuente de los datos: Dialnet