Ocratoxina A en plasma humanonuevos datos de exposición en España

  1. Pérez de Obanos, A.
  2. López de Ceraín, A.
  3. Jiménez, A.M.
  4. González Peñas, E. 1
  5. Bello, J.
  1. 1 Universidad de Navarra
    info

    Universidad de Navarra

    Pamplona, España

    ROR https://ror.org/02rxc7m23

Revue:
Revista de toxicología

ISSN: 0212-7113

Année de publication: 2001

Volumen: 18

Número: 1

Pages: 19-23

Type: Article

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D'autres publications dans: Revista de toxicología

Résumé

In order to study the occurrence of Ochratoxin A in a southern region of Spain, Granada, 83 blood plasma samples collected from women have been analysed. The method of analysis is based on a liquid-liquid extraction followed by a High Performance Liquid Cromatographic (HPLC) analysis with fluorescence detection. The method has been validated and a detection limit of 0.21 ng/mL has been obtained. Ochratoxin A has been detected in 72% of the samples. The mean value was 0.63 ng/mL, with a standard deviation of 0.41 ng/mL and a range of concentrations from 0.11 ng/mL to 6.96 ng/mL. These results are similar to those found in other northern and medium regions of Spain, and to those found in the surrounding countries. Statistical analysis by Kruskal-Wallis (n = 70; χ2= 4.591; p = 0.597), did not show any difference between plasma levels and the season when the samples were collected. These findings prove the general and wide exposition to Ochratoxin A of Spanish population, although the Daily Ochratoxin A Intake calculated from the mean value obtained in this study, is lower than the maximum level recommended by the Joint FAO/WHO Expert Committee on Food Additives.

Références bibliographiques

  • López de Cerain, A., Jiménez, A.M., Ezpeleta, O., Bello, J., Efectos tóxicos de la Ocratoxina A (2000) Revista de Toxicología, 17, pp. 61-69
  • Ochratoxin A (1993), 56, pp. 489-521. , Some naturally occurring substances: food items and constituents, heterocyclic aromatic amines and mycotoxins. IARC monographs on the evaluation of carcinogenic risks to humans
  • Krogh, P., Elling, F., Fungal toxins and endemic (Balkan) nephropathy (1976) The Lancet, JULY, pp. 3-40
  • Barnes, J.M., Carter, R.L., Peristianis, G.C., Austwick, P.K.C., Flynn, F.V., Aldridge, W.N., Balkan (endemic) nephropathy and a toxin-producing strain of Penicillium verrucosum var. cyclopium: An experimental model in rats (1977) The Lancet, 26, pp. 671-675
  • Pfohl-Leszkowicz, A., Grosse, Y., Kane, A., Creppy, E.E., Dirheimer, G., Differential DNA adduct formation and disappearance in three mouse tissues after treatment with the mycotoxin Ochratoxin A (1993) Mutation Research, 289, pp. 265-273
  • Fóllmann, W., Hillebrand, I.E., Creppy, E.E., Bolt, H.M., Sister chromatid exchange in cultured isolated porcine urinary bladder epithelial cells (PUBEC) treated with Ochratoxin A and alpha (1995) Archives of Toxicology, 69, pp. 280-286
  • Obrecht-Pflumio, S., Grosse, Y., Pfohl-Leszkowicz, A., Dirheimer, G., Protection by indomethacin and aspirin against genotoxicity of Ochratoxin A, particularly in the urinary bladder and kidney (1996) Archives of Toxicology, 70, pp. 244-248
  • Degen, G.H., Gerber, M.M., Obrecht-Pflumio, S., Dirheimer, G., Induction of micronuclei with Ochratoxin A in ovine seminal vesicle cell cultures (1997) Archives of Toxicology, 71, pp. 365-371
  • Dörrenhaus, A., Föllmann, W., Effects of Ochratoxin A on DNA repair in cultures of rat hepatocytes and porcine urinary bladder epithelial cells (1997) Archives of Toxicology, 71, pp. 709-713
  • Dirheimer, G., Recent advances in the genotoxicity of mycotoxins (1998) Revue Médicale Véterinaire, 149, pp. 605-616
  • Flieger, A., Reckwitz, T., Golka, K., Sculze, H., Föllmann, W., Bolt, H.M., Genotoxicity of the mycotoxin Ochratoxin A detected by UDS in primary cultures of human urothetial cells (1998) Toxicology Science, 42 (1 S), p. 79. , abstract
  • Jiménez, A.M., López de Cerain, A., González-Peñas, E., Bello, J., Betbeder, A.M., Créppy, E.E., Exposure to ochratoxin A in Europe: Comparison with a region of northern Spain (1998) Journal of Toxicology-Toxins Reviews, 17, pp. 479-491
  • Burdaspal, P.A., Legarda, T.M., Datos sobre presencia de ocratoxina A en plasma humano en España (1998) Alimentaria, MAYO, pp. 103-109
  • Bauer, J., Gareis, M., Ochratoxin A in the food-chain (1987) Journal of Veterinary Medicine Series B, 34, pp. 613-627
  • Jiménez, A.M., López de Cerain, A., González-Peñas, E., Bello, J., A high performance liquid chromatographic method for the determination of Ochratoxin A in human plasma (1999) Chromatographia, 50, pp. 457-460
  • Bressolle, F., Bromet-Petit, M., Audran, M., Validation of liquid chromatographic and gas chromatographic methods. Application to pharmacokinetics (1996) Journal of Chromatography B, 686, pp. 3-10
  • Creppy, E.E., Betbeder, A.M., Gharbi, A., Counord, J., Castegnaro, M., Bartsch, H., Moncharmont, P., Dirheimer, G., Human ochratoxicosis in France (1991) Mycotoxins, Endemic Nephropathy and Urinary Tract Tumours, p. 145. , M Castegnaro, R Plestina, G Dirheimer, IN Chernozemsky, H Bartsch. Lyon: IARC Science Publications No.115
  • Palli, D., Miraglia, M., Saieva, C., Masala, G., Cava, E., Colatosti, M., Corsi, A.M., Brera, C., Serum levels of ochratoxin A in healthy adults in Tuscany: Correlation with individual characteristics and between measurements (1999) Cancer, Epidemiology, Biomarkers and Prevention, 8, pp. 265-269
  • Fuchs, R., Radic, B., Ceovic, S., Sostaric, B., Hult, K., Human exposure to ochratoxin A (1991) Mycotoxins, Endemic Nephropathy and Urinary Tract Tumours, p. 131. , M. Castegnaro, R. Plestina, Dirheimer G. Chernozemsky IN, y Bartsch H, editores. Lyon: IARC Science publications No. 115
  • Ruprich, J., Ostry, V., Study of human exposure to ochratoxin A and assessment of possible sources (1993) Central European Journal of Public Health, 1, pp. 46-48
  • Frohlich, A.A., Marquardt, R.R., Ominsky, K.H., Ochratoxin A as a contaminant in the human food chain: A canadian perpective (1991) Mycotoxins, Endemic Nephropathy and Urinary, tract Tumours, p. 139. , Castegnaro M, Plestina R, Dirheimer G, Chernozemsky In Y Bartsch H. Editores. Lyon: IARC Science publications No.115
  • Smith, J.E., Lewis, C.W., Anderson, J.G., Solomons, G.L., (1994) The penicillium toxins-pathology and epidemiology, p. 111. , Goulden ML. Mycotoxins in Human Nutrition and Health. Agro-Industrisl Research Division, DG xii. European Comission, EUR16048EN
  • Breiholtz, A., Olsen, M., Dahläck, A., Kult, K., Plasma Ochratoxin A levels in three swedish populations surveyed using an ion-pair HPLC technique (1991) Food Additives and Contaminants, 8, pp. 183-186
  • Hagelberg, S., Fuchs, P., Hult, K., Toxicokinetics of Ochratoxin A in several species and its plasma-binding properties (1989) Journal of Applied Toxicology, 9, pp. 91-96
  • Klaassen, C.D., Distribution, excretion and absorption of toxicants (1986) Casarett and Doull's Toxicology, pp. 33-63. , The Basic Science of Poisons, Klaassen, Amdur y Doull (Eds.), 3a edición, Nueva York. Mc Millan
  • (1995) Joint FAO/WHO Expert Committee on Food Additives, 44th Meeting, , Technical Report Series N° 859, Ginebra, OMS
  • Olsen, M., Thorup, I., Knudsen, I., Larsen, J.J., Hald, B., Olsen, J., (1991) Health evaluation of Ochratoxin A in food products, 545. , Nordiske Seminar-og Arbejdsrapporter, Copenhagen, Nordic Council of Ministers
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