Source Data Files from publication: A Protein Misfolding Shaking Amplification-based method for the spontaneous generation of hundreds of infectious prions, Nature Communications 2024

  1. Eraña, Hasier 123
  2. Sampedro Torres Quevedo, Cristina 3
  3. Charco, Jorge M. 234
  4. Díaz Domínguez, Carlos M. 23
  5. Peccati, Francesca 3
  6. San-Juan-Ansoleaga, Maitena 3
  7. Vidal, Enric 56
  8. Gonçalves-Anjo, Nuno 3
  9. Pérez-Castro, Miguel A. 3
  10. González-Miranda, Ezequiel 3
  11. Piñeiro, Patricia 3
  12. Fernández-Veiga, Leire 3
  13. Galarza-Ahumada, Josu 3
  14. Fernández-Muñoz, Eva 3
  15. Perez de Nanclares, Guiomar 78
  16. Telling, Gelnn C. 9
  17. Geijo, María V. 10
  18. Jiménez-Osés, Gonzalo 3
  19. CASTILLA, JOAQUÍN 2311
  1. 1 Atlas Molecular Pharma (Spain)
  2. 2 Centro de Investigación Biomédica en Red de Enfermedades infecciosas (CIBERINFEC)
  3. 3 Centro de Investigación Cooperativa en Biotecnología
    info

    Centro de Investigación Cooperativa en Biotecnología

    Zamudio, España

  4. 4 ATLAS Molecular Pharma (Spain)
  5. 5 Centre Recerca en Sanitat Animal
    info

    Centre Recerca en Sanitat Animal

    Bellaterra, España

    ROR https://ror.org/011jtr847

  6. 6 Institute of Agrifood Research and Technology
  7. 7 Hospital Universitario Araba sede Txagorritxu
  8. 8 Instituto de Investigación Sanitaria Biobizkaia
    info

    Instituto de Investigación Sanitaria Biobizkaia

    Barakaldo, España

    ROR https://ror.org/0061s4v88

  9. 9 Colorado State University
    info

    Colorado State University

    Fort Collins, Estados Unidos

    ROR https://ror.org/03k1gpj17

  10. 10 Instituto Vasco de Investigación y Desarrollo Agrario
    info

    Instituto Vasco de Investigación y Desarrollo Agrario

    Derio, España

    ROR 03rf31e64

  11. 11 Ikerbasque
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Verleger: Zenodo

Datum der Publikation: 2024

Art: Dataset

DOI: 10.5281/ZENODO.10579517 lock_openOpen Access editor

Zusammenfassung

This Dataset contains all the Source Data corresponding to the results from the manuscript entitled "A Protein Misfolding Shaking Amplification-based method for the spontaneous generation of hundreds of infectious prions", accepted for publication in Nature Communications Journal. The dataset includes uncropped scans of all gels and blots used to generated the final figures and tables (including Supplementary figures and tables), as well as the raw data used to generate the plots shown in the manuscript. The dataset is organized by Figures and Tables, with the source data files containing all the information used in each figure or table included in a compressed (.zip) file. The figure and table numbering used in the manuscript is kept, with source data available for: Figures 1 & 2, Figure 3, Supplementary figure 1, Supplementary figure 2,  Supplementary figure 3, and Supplementary figure 4. Manuscript abstract: Prion diseases are a group of rapidly progressing neurodegenerative disorders caused by the misfolding of the endogenous prion protein (PrPC) into a pathogenic form (PrPSc). This process, despite being the central event underlying these disorders, remains largely unknown at a molecular level, precluding the prediction of new potential outbreaks or interspecies transmission incidents. In this work, we present a method to generate infectious bona fide recombinant prions de novo, allowing a comprehensive analysis of protein misfolding across a wide range of prion proteins from mammalian species. We studied more than 380 different prion proteins from mammals and classified them according to their spontaneous misfolding propensity and their conformational variability. This study aims to address fundamental questions in the prion research field such as defining infectivity determinants, interspecies transmission barriers or the structural influence of specific amino acids and provides invaluable information for future diagnosis and therapy applications.