Structural study of CUG-repeating small RNAs complexed with silencing suppressor P19

  1. Katorcha ---, Elizaveta
Supervised by:
  1. Lioudmila Malinina Director

Defence university: Universidad del País Vasco - Euskal Herriko Unibertsitatea

Fecha de defensa: 07 March 2012

Committee:
  1. Félix María Goñi Urcelay Chair
  2. Marcelo Eduardo Guerin Secretary
  3. Miquel Coll Capella Committee member
  4. Josefa de Lourdes Campos Lopez Committee member
  5. Juan Antonio Subirana Torrent Committee member

Type: Thesis

Teseo: 115719 DIALNET lock_openADDI editor

Abstract

The study is focused on structural aspects of interaction between silencing suppressor p19 and CUG-repeating small RNAs. The work involves crystal structure determination of a protein-unbound RNA form and RNA fragments of various lengths (19, 20, 21 nucleotides) complexed with p19-suppressor. Results prove the ability of silencing suppressor p19 to bind CUG-repeating small RNAs, as well as reveal features of U•U mismatches flanked by Watson-Crick C•G base pairs in p19-bound and p19-unbound states. In addition, structural data reveal a p19 specific site for anchoring extra nucleotides in small RNAs. In general, the study extends our knowledge about the mechanism of small RNA recognition by silencing suppressor p19.