Structural study of CUG-repeating small RNAs complexed with silencing suppressor P19
- Katorcha ---, Elizaveta
- Lioudmila Malinina Directeur/trice
Université de défendre: Universidad del País Vasco - Euskal Herriko Unibertsitatea
Fecha de defensa: 07 mars 2012
- Félix María Goñi Urcelay President
- Marcelo Eduardo Guerin Secrétaire
- Miquel Coll Capella Rapporteur
- Josefa de Lourdes Campos Lopez Rapporteur
- Juan Antonio Subirana Torrent Rapporteur
Type: Thèses
Résumé
The study is focused on structural aspects of interaction between silencing suppressor p19 and CUG-repeating small RNAs. The work involves crystal structure determination of a protein-unbound RNA form and RNA fragments of various lengths (19, 20, 21 nucleotides) complexed with p19-suppressor. Results prove the ability of silencing suppressor p19 to bind CUG-repeating small RNAs, as well as reveal features of U•U mismatches flanked by Watson-Crick C•G base pairs in p19-bound and p19-unbound states. In addition, structural data reveal a p19 specific site for anchoring extra nucleotides in small RNAs. In general, the study extends our knowledge about the mechanism of small RNA recognition by silencing suppressor p19.